Institute of Convergence Science, Yonsei University

다음과 같이 기초나노의학 연구센터(센터장: 화학과 천진우 교수)에서

Prof. Shan Xiang Wang을 모시고

세미나를 개최합니다.

참석하셔서 융합연구에 관한 좋은 아이디어를 발전시키는 기회가 되시길 빕니다.

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미래융합연구원 기초나노의학 연구센터 세미나 개최

일시: 2013년 9월 9일 (월) 오후 2:30~3:30

장소: 미래융합연구원ICONS Room (학술정보관 721호)

연사: Prof. Shan Xiang Wang

Materials Science and Engineering, School of Engineering, Stanford University

주관 : 기초나노의학 연구센터(센터장 천진우 화학과 교수) 

제목: Magneto-Nano Chips for Ultrasensitive and Multiplex Detection of Protein Biomarkers of Tumor

Abstract:

Magneto-Nano Chips for Ultrasensitive and Multiplex Detection of Protein Biomarkers of Tumor

Shan X. Wang1,2 a

1Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305 USA

2Department of Electrical Engineering, Stanford University, Stanford, CA 94305 USA

asxwang@stanford.edu

Abstract: Reproducible and multiplex protein assays are greatly desired by cancer biologists as well as clinical oncologists to rapidly follow numerous proteins in clinical samples. We have now successfully applied magneto-nano biochips based on giant magnetoresistance (GMR) spin valve sensor arrays and magnetic nanoparticle labels (nanotags) to the detection of biological events in the form of multiplex protein assays (4-to 64-plex) with great speed (0.5 ? 2 hours), sensitivity (1 picogram/ml concentration levels or below), selectivity, and economy [1]. The technology platform is well suited for longitudinal studies of animal or human cohorts after therapeutic intervention or radiation exposure [2]. Very recently, the magneto-nanosensor is paired with a micromachined magnetic separation device, magnetic sifter [3], to isolate circulating tumor cells in lung cancer patients and then to detect the EGFR mutation status using mutation specific antibody probes.

We have also achieved the first demonstration of a nanolabel-based technology capable of rapidly isolating cross-reactive antibody binding events in a highly multiplex manner. By combining magnetic nanotechnology with immunology, we have devised an easy to use and rapid auto-assembly assay which is ideal for high-density screens of aberrant protein binding events [4]. Such a technology has the potential to revolutionize the current practices in the proteomics and drug development community by providing researchers with the tools to rapidly investigate both on and off-target protein binding events. Furthermore, this technology is more sensitive and specific than label-free technologies (e.g., Surface Plasmon Resonance (SPR) based approaches such as Biacore), can be scaled up more readily, and consumes far less valuable reagents. It is also possible to combine the magneto-nanosensor array with nucleic acid programmable protein array (NAPPA) array [5] to study protein-protein interactions of thousands of targets in a one-pot experiment. Finally, magneto-nanosensors have been extended to advanced reverse phase assay format in which multiple samples and multiple targets can be interrogated on a single chip.

References:

[1]    Gaster RS, Hall DA, et al., Nature Medicine, 15, 1327-1332, 2009.

[2]    Kim D et al., Scientific Reports, 3, 2234, 2013.

[3]    Wang SX et al., Magnetic sifter, US Patent No. 7,615,382; issued Nov. 10, 2009.

[4]   Gaster RS, Xu L, Han SJ, et al., Nature Nanotechnology, 6, 314-320, 2011.

[5]    Lee J et al., Expert Review of Proteomics, 10(1), 65-75, 2013.

Shan X. Wang’s brief biography

   Dr. Wang is a Professor of Materials Science & Engineering and jointly of Electrical Engineering at Stanford University, and by courtesy, a Professor of Radiology at Stanford School of Medicine, and the Director of Center for Magnetic Nanotechnology. He also serves as a co-principal investigator of the Center for Cancer Nanotechnology Excellence and Translation (CCNE-T) at Stanford University. He has over 200 publications, and holds 38 issued or pending patents in the area of magnetic nanotechnology, biosensors, spintronics, power management and information storage. He is a Fellow of the Institute of Electrical and Electronics Engineers (IEEE) and a Fellow of American Physical Society (APS). The international recognition of his work has led to well over 100 invitations to speak or lecture in major conferences and institutions. Dr. Wang received the Ph.D. in Electrical and Computer Engineering from the Carnegie Mellon University in1993.